Diagnostic value of hemomyelogram for acute appendicitis / 南方医科大学学报

<p><b>OBJECTIVE</b>To analyze the relationship between hemomyelogram and sererity of acute appendicitis and identify the best routine blood test feature for perforation.</p><p><b>METHODS</b>721 patients were included in this study, all of whom underwent appendectomy for the clinical diagnosis of appendicitis during the years of 2010-2013. The initial preoperative hemomyelogram was evaluated at different stages of appendicitis. The area under the ROC curve was used to assess the clinical feature with greater diagnostic accuracy of perforation. Total lymphocyte counts of 1.83 was used in the prediction of perforative appendicitis. A group of 467 patients was used for validation to confirm the diagnostic value of the cut-off value.</p><p><b>RESULTS</b>The percentage of lymphocytes had the closest association with the evolutionary phase of acute appendicitis. Total lymphocyte counts < or=1.83 indicated perforation, with high sensitivity and low specificity.</p><p><b>CONCLUSION</b>The percentage of lymphocytes and total lymphocyte counts are helpful as a diagnostic paramete for different stages of acute appendicitis.</p>

Feasibility of continuous extracorporeal normothermic liver perfusion using autologous blood: a study in pigs / 南方医科大学学报

<p><b>OBJECTIVE</b>To study the feasibility of sustaining the viable status of a liver graft in at least 96 h by extracorporeal perfusion using autologous blood.</p><p><b>METHODS</b>Eight extracorporeal porcine liver perfusions using autologous blood were performed, each for 96 h with hepatectomy, cold preservation, cannulation of vessels, and initiation of perfusion with normothermic oxygenated porcine blood. The graft viability was assessed by metabolic, synthetic, hemodynamic, and histologic parameters.</p><p><b>RESULTS</b>After 96 h of normothermic, extracorporeal perfusion using autologous blood, the isolated livers maintained normal physiological levels of pH and electrolytes with sustained hepatic protein synthesis (complement and factor V) throughout the perfusion. Hemodynamic parameters maintained normal physiological ranges. Histological inspection demonstrated good preservation of the liver with a good architectural integrity.</p><p><b>CONCLUSION</b>It is possible to sustain the viable status of a liver graft within 96 h by extracorporeal perfusion using autologous blood.</p>